Journal: Molecular neurobiology

Article Title: MicroRNA Profiling Identifies miR-196a as Differentially Expressed in Childhood Adrenoleukodystrophy and Adult Adrenomyeloneuropathy

doi: 10.1007/s12035-016-9746-0

Figure Lengend Snippet: Differentially expressed miRNA in cALD (grey) and AMN (white) fibroblasts from miRNome miRNA PCR array. The expression of 1008 most abundantly expressed and best characterized miRNA sequences in the human miRNA genome was profiled using Human miRNome miRNA PCR array (Qiagen-MIHS-216Z) in triplicate. The relative expression was calculated using the ΔΔCT method and is presented as fold change relative to control. Among 1008 miRNAs, 11 miRNAs were significantly differentially regulated between cALD and AMN as shown in the figure. miR-196a was most significantly differentially regulated

Article Snippet: To examine the possible role of microRNA (miRNA) in X-ALD disease mechanisms for differences in cALD and AMN phenotype, we profiled 1008 known miRNA in cALD, AMN, and normal human skin fibroblasts using miScript miRNA PCR array (Qiagen) and selected miRNAs which had differential expression in cALD and AMN fibroblasts.

Techniques: Expressing

Journal: Molecular neurobiology

Article Title: MicroRNA Profiling Identifies miR-196a as Differentially Expressed in Childhood Adrenoleukodystrophy and Adult Adrenomyeloneuropathy

doi: 10.1007/s12035-016-9746-0

Figure Lengend Snippet: Validation of miR-196a expression in human skin fibroblasts (a) and human brain samples (b) by qRT-PCR. miR-196a expression was validated in human skin fibroblasts and human brain samples (from equivalent brain regions) using Qiagen miScript primer assays by qRT-PCR. The values were normalized to SNORD61 and presented as fold change. As represented by figure, miR-196a expression was downregulated in cALD fibroblasts and brain samples and upregulated in AMN fibroblasts and brain samples. B3 is brain sample from AMN that subsequently developed CNS disease. Downregulation of miR-196a expression in brain sample B3 indicates of cerebral involvement. Thirty-five percent of AMN patients develop cerebral involvement

Article Snippet: To examine the possible role of microRNA (miRNA) in X-ALD disease mechanisms for differences in cALD and AMN phenotype, we profiled 1008 known miRNA in cALD, AMN, and normal human skin fibroblasts using miScript miRNA PCR array (Qiagen) and selected miRNAs which had differential expression in cALD and AMN fibroblasts.

Techniques: Expressing, Quantitative RT-PCR

Journal: Molecular neurobiology

Article Title: MicroRNA Profiling Identifies miR-196a as Differentially Expressed in Childhood Adrenoleukodystrophy and Adult Adrenomyeloneuropathy

doi: 10.1007/s12035-016-9746-0

Figure Lengend Snippet: Roles of miR-196a in target gene expression. To establish the regulation of target genes by miR-196a, miR-196a mimic was transfected in ABCD1 knockdown U87 astrocytes cells (U87 Lenti) and transfection efficiency evaluated by miScript qRT-PCR. SNORD61 was used as an internal control and represented as miR-196a/SNORD61 (a). The expression of target genes IKKα and IKKβ was analysed after the transfection of miR-196a mimic and normalized to GAPDH. The expression of these genes was significantly increased in ABCD1 silenced cells as compared to wild type but significantly decreased after transfection of mimic in U87 Lenti cells (b and c). **P < 0.05. An unpaired student t test was used to determine the statistical significance of the data from three independent experiments

Article Snippet: To examine the possible role of microRNA (miRNA) in X-ALD disease mechanisms for differences in cALD and AMN phenotype, we profiled 1008 known miRNA in cALD, AMN, and normal human skin fibroblasts using miScript miRNA PCR array (Qiagen) and selected miRNAs which had differential expression in cALD and AMN fibroblasts.

Techniques: Expressing, Transfection, Quantitative RT-PCR